From December 2020 through April 2023, the National LGBTQIA+ Health Education Center developed and facilitated three HIV prevention learning collaborative cohorts involving 29 organizations and 79 participants. Participants represented a range of role groups within health centers and worked in diverse locations around the country. Overall, participants described a significant amount of knowledge gained from the sessions and satisfaction with the training. Two of the cohorts overlapped with the Covid-19 pandemic; the learning collaborative’s virtual format was conducive to pandemic limitations on in-person gatherings, and the program provided a strategy to continue building capacity for PrEP during and despite the pandemic. Key lessons from the learning collaborative program include the importance of training about logistical and programmatic aspects of PrEP, the feasibility and desirability of engaging multidisciplinary teams in PrEP training, balancing education about scientific advancements in PrEP with content on implementation and addressing HIV risk perception and adherence. Going forward, we aim to create new content on risk perception and adherence and institute systematic evaluations of the learning collaborative cohorts to facilitate ongoing innovation and improvement.