This toolkit focuses on the implementation stage of training sites to successfully engage and retain LGBTQIA+ participants. Within the toolkit, you will find information on foundational LGBTQIA+ concepts and terminology, best practices for affirming transgender and gender diverse participants, and recommendations for collecting SOGI data as part of participant demographics. By learning these competencies, all study staff can become skilled and confident in supporting the dignity of LGBTQIA+ participants, and ultimately, can contribute to improving health equity for LGBTQIA+ communities.